Early service . From its formation through December 1864, the regiment were primarily tasked with guard duty on the Nashville & Northwestern Railroad, During this period, the regiment was engaged in a skirmish along the railroad on September 4, 1864, and would later take part in the
Battle of Johnsonville from November 4-5, 1864.
Battle of Nashville In December 1864, the regiment was attached to the 2nd Colored Brigade of the District of Etowah. During the
Battle of Nashville, the 100th USCI participated in the Union defense and the subsequent assault against the
Confederate Army of Tennessee. On the second day of the battle, the regiment was involved in heavy fighting at Overton Hill. Following the Union victory, the regiment would join the pursuit of General
John Bell Hood's forces toward the
Tennessee River, which lasted from December 17, to December 28, 1864. In January 1865, the regiment returned to its previous assignment of guarding the Nashville and Northwestern Railroad. It remained on this duty for the duration of 1864 as the war concluded. The 100th USCI was mustered out of Service in
Nashville, Tennessee on December 26, 1865 == Notable commanders ==