• Colonel Samuel Croasdale – killed in action at the Battle of Antietam • Colonel Joseph A. Mathews – captured at the Battle of Chancellorsville •
Lieutenant Colonel William W. Hammersly – commanded at the Battle of Antietam after Col. Croasdale was killed in action until wounded in action; resigned January 1863 due to his wounds received at Antietam •
Major Joel B. Wanner – commanded at the Battle of Antietam after Ltc. Hammersly was wounded in action ==Notable members==