It was established on 1 April 2014, mainly from volunteers and operated mainly in the Southern and Mariupol directions during the
War in Donbass participating in the liberation of
Shyrokyne,
Pavlopil and
Pyshchevyk. On 20 June 2015, two soldiers of the battalion, Nazarenko Andriy Volodymyrovych and Miloserdov Volodymyr Oleksandrovych were killed in a clash with a separatist DRG between
Chermalyk and the
Pavlopil. On 18 July 2015, a soldier of the battalion, Ryzhov Stanislav Anatoliyovych was killed by a landmine while performing a reconnaissance mission near
Mariupol in Oktiabr. On 15 December 2015, while performing a combat reconnaissance mission near Pavlopil, a reconnaissance group of the battalion came under sniper fire killing one soldier, Nosenko Artem Vyacheslavovych. On 7 July 2016, near
Lopaskyne, a Volkswagen minibus of the battalion was blown up by a landmine, two soldiers of the battalion, Kovalskyi Anatoliy Viktorovych and Yablonsky Mykola Mykolayovych were killed. On 30 August 2016, a soldier of the battalion, Zhulynsky Mykola Volodymyrovych was killed by a Separatist sniper while performing a combat mission near Dovhe. On 11 November 2016, a soldier of the battalion, Oleg Olegovich Suprikin was killed while performing a combat operation in
Malinove. On 30 April 2017, while performing a combat mission near Katerynivka, Popasnyanskyi district, the battalion personnel came under heavy mortar shelling from Molodizhne, a soldier, Mayboroda Denis Anatoliyovych was killed and two more were wounded. On 13 December 2017, a soldier of the battalion, Kornelyuk Pavlo Volodymyrovych was killed by a Separatist sniper at an observation post in
Kruta Balka. A soldier of the battalion, Panin Viktor Vyacheslavovich, was killed in
Avdiivka on 21 February 2018. On 10 March 2020, at a position near
Pisky, separatists'
ATGMs hit a
GAZ-66 of the battalion, two soldiers of the battalion, Soltys Viktor Mykolayovych and Andriy Oleksandrovych Vedeshin were killed and eight more were wounded, an additional soldier, Perezhogin Ilya Nikolaevich was killed by a sniper attack at the same position. On 23 August 2021, a soldier of the battalion, Aksyonov Alexander Vasilyevich was killed as a result of separatist shelling on a stronghold. Since the beginning of the
2022 Russian invasion of Ukraine, the battalion has actively participated in the
Southern Ukraine campaign performing defensive, assault, reconnaissance, and other special tasks. In early March 2022, a reconnaissance group of the battalion came under enemy fire near
Luhove and a soldier, Obertiy Mykola Grigorovich went
MIA and was later confirmed dead. A soldier of the battalion, Petruk Valentyn Vasylovich was killed in action on 23 April 2022 in
Pokrovsk. An officer of the battalion, Gutnik Vadim Svyatoslavovych was killed in combat as a result of shelling on 26 July 2022. In September 2022, a reconnaissance group of the battalion successfully adjusted artillery and aviation fire on a Russian troop concentration in
Mala Seidemynukha destroying equipment and personnel. It also engaged in small arms battles prompting Russian forces to withdraw. On 10 November 2022, the battalion liberated
Snihurivka in the
Mykolaiv Oblast. On 6 September 2023, a soldier of the battalion, Orlov Andriy Igorovych (
Hero of Ukraine) was killed in combat on the frontlines. On 1 October 2023, the 131st Separate Reconnaissance Battalion was awarded the honorary name "Colonel Yevhen Konovalets". A soldier of the battalion, Kryshtov Ivan Igorovich was killed by shelling on 29 July 2024. ==Commanders==