The route runs almost entirely along Mission Street between the
San Francisco Ferry Building Plaza and Mission and San Jose Streets in Daly City. Outbound buses run on Otis Street for the one-way segment of Mission near the
Central Freeway. At in length, the 14 Mission is Muni's longest trolleybus line. The route operates 24 hours as part of the
All Nighter network.
Mission Rapid in June 2018 Limited-stop service is provided by the 14R Mission Rapid between 5th Street and Sickles Avenue/Acton Street in order to provide faster service through the corridor. The southern terminus is
Daly City BART station. This service utilizes regular buses in order to allow passing of local services and due to lack of
overhead line at the southern terminus.
Mission Express The 14X Mission Express was a modified rush-hour route that was primarily operated on Mission Street, but ran
express between Trumbull and 6th Streets, partially utilizing
Interstate 280 and
U.S. Route 101. This service utilized regular buses due to lack of
overhead line on the non-Mission segments. The 14X was suspended as part of the city's
COVID-19 response in April 2020, and remains temporarily out of service as of 2025. ==History==