Elections were held in the colony of Queensland between 27 April 1860 to 11 May 1860 to elect all 26 members of the colony's Legislative Assembly. It was conducted in nine single-member constituencies, four two-member constituencies, and three three-member constituencies, all with a first past the post system. Suffrage was limited to men who owned property. The electorates were also malapportioned to favour pastoral districts. This was the first election after the separation of Queensland in December 1859.