The 1966 Wyoming Cowboys football team was an American football team that represented the University of Wyoming as a member of the Western Athletic Conference (WAC) during the 1966 NCAA University Division football season. In their fifth year under head coach Lloyd Eaton, the Cowboys compiled a 10–1 record, won the first of three consecutive WAC titles, outscored opponents by a total of 355 to 89, and had the nation's best rushing defense.