The 2000–01 Oklahoma State Cowboys basketball team represented Oklahoma State University as a member of the Big 12 Conference during the 2000–01 NCAA Division I men's basketball season. They were led by 11th-year head coach Eddie Sutton and played their home games at Gallagher-Iba Arena in Stillwater, Oklahoma. They finished the season 20–10, 10–6 in Big 12 play to finish in fifth place. The Cowboys lost to Missouri in the quarterfinals of the Big 12 tournament. The team received an at-large bid to the NCAA tournament as the No. 11 seed in the East region. Oklahoma State lost to No. 6 seed USC in the opening round.