The 2001 Oklahoma State Cowboys football team represented Oklahoma State University as a member of the Big 12 Conference during the 2001 NCAA Division I-A football season. Led by first-year head coach Les Miles, the Cowboys compiled an overall record of 4–7 with a mark of 2–6 in conference play, placing fifth in the Big 12's South Division. Oklahoma State played home games at Lewis Field in Stillwater, Oklahoma.