Twenty-seven teams qualified for the tournament, with five qualifying directly to the round of 16. The remaining 22 teams enter in Round one. In addition to national league competitions in the United States, Mexico, and Canada, 12 of 27 berths were determined by international competitions, including the
2025 Leagues Cup (three),
2025 CONCACAF Caribbean Cup (three), and
2025 CONCACAF Central American Cup (six). Two
Canadian Premier League teams qualified automatically, while the United States-only
2025 U.S. Open Cup knockout competition and Canada-only equivalent, the
2025 Canadian Championship, provided one berth each. The winners of the three international competitions, along with the winner of
MLS Cup 2025 and champion of the
2024–25 Liga MX season, received the five byes into the round of 16. {{location map+|CONCACAF|width=680
Distribution Twenty-seven teams (from ten associations) qualified for the tournament. •
North American Zone: 18 teams (from three associations) •
Central American Zone: 6 teams (from four associations) •
Caribbean Zone: 3 teams (from three associations) Five teams received a bye into the round of 16. • The champion of the regional competitions for each of
CONCACAF's constituent zones; North American, Central American, and Caribbean • The champions of
Liga MX of Mexico and
Major League Soccer of the United States and Canada (Liga MX had a split Apertura and Clausura system, so the champion team with the higher aggregate points took this place. If a team won both Apertura and Clausura, they took this place.) The remaining 22 teams enter in Round one. • The runners-up and third-place team from the MLS–Liga MX
Leagues Cup • The runners-up and third-place team from the
Caribbean Cup • The runners-up, remaining semi-finalists, and two play-in winners from the
Central American Cup • The
U.S. Open Cup winners • The
Canadian Championship winners • The
Canadian Premier League regular season winners and playoff champions • The remaining winner with the worse record, two runners-up, and the two best next clubs by season aggregate from
Liga MX • The
MLS Supporters' Shield winners • The remaining MLS Conference regular-season leader, and the next two teams in the Supporters' Shield table ==Teams==