The first round qualifying fixtures were played on the weekend of 12 September 2009, with replays being played the following mid-week. A total of 232 clubs took part in this stage of the competition, including the 167 winners from the preliminary round and 65 entering at this stage from the top division of the three leagues at
Level 7 of English football, while
Halesowen Town from Southern League Premier Division was barred from FA Cup because of financial difficulties. The round featured 17 clubs from Level 10 still in the competition, being the lowest-ranked clubs in this round. As
Newcastle Blue Star folded after the draw took place,
Ossett Albion were given a walkover to the second round qualifying.
Bobby Traynor was voted Player of the Round for his hat-trick in
Kingstonian's 4–1 win against
Bognor Regis Town. ==Second round qualifying==