Format The relegation play-offs (
play-out in Italian) is contested over two legs between the teams finishing 16th and 17th in the regular season. The higher-placed team hosts the second leg. If the teams are level on aggregate score, the lower-placed team in the regular season standings is relegated to
Serie C. However, if the two teams finished tied on points in the regular season, extra time and, if necessary, a penalty shoot-out are used to determine the winner.
Overview The relegation play-out was originally scheduled for 19 and 26 May 2025 between Frosinone and Salernitana, who finished in 16th and 17th place respectively. Sampdoria were in 18th place and due to be directly relegated, while Brescia ended up in 15th place, securing their status in Serie B. However, on 18 May 2025,
Lega B announced the postponement of the fixtures after the
FIGC’s Federal Public Prosecutor’s Office had concluded its investigation into financial irregularities concerning Brescia. As the investigation could lead to a points deduction that would alter the final standings, the league opted to delay the play-out pending the outcome of the disciplinary proceedings. On 26 May,
Lega B decided that the relegation play-offs would likely be scheduled for 15 and 20 June 2025. Brescia were deducted four points for administrative violations on 29 May 2025. The penalty altered the final standings: Brescia dropped into the relegation zone, Frosinone moved up to 15th place and avoided relegation, and the play-out positions became Salernitana (16th) and Sampdoria (17th). Brescia initially appealed the deduction, but on 9 June 2025 the club announced it would renounce the appeal, effectively accepting the four-point penalty and the resulting relegation to Serie C. With the deduction confirmed and Brescia’s appeal withdrawn, the revised standings were definitive. Lega B subsequently rescheduled the play-out for June, with the second leg confirmed for 22 June 2025.
First leg Second leg Sampdoria won 5–0 on aggregate and remained in Serie B, while Salernitana were relegated to Serie C. The second leg in Salerno was suspended in the 74th minute with Sampdoria leading 0–2 due to incidents caused by Salernitana fans. Lega B awarded Sampdoria a 3–0 win for the second leg, giving them a 5–0 aggregate victory. ==Season statistics==