The winner of the
2024–25 Slovak Cup,
Spartak Trnava, finished in the top 3 in the league, which already secured them a spot in the
Europa League. As a result, a playoff was held for the final spot in the Conference League, which involved the teams ranked in 4th–7th place at the conclusion of the regular season. The play-offs were one-off matches with extra time and penalties used to determine the winner if necessary, with the higher-ranked teams given home advantage against the lower-ranked teams (i.e.
3rd v. 7th and
5th v. 6th) in the semi-finals. The higher-ranked of the two semi-final winners also gained home advantage in the final.
DAC Dunajská Streda won the play-offs but were subsequently excluded from the Conference League due to the club having the same ownership as Hungarian side
Győri ETO FC, who had also qualified for the competition.
Košice took their place in Conference League qualifying.
Semi-finals Final ==Relegation play-offs==