Defending champion Camila Osorio defeated Katarzyna Kawa in the final, 6–3, 6–3 to win the singles tennis title at the 2025 Copa Colsanitas. Osorio was the second player, after countrywoman Fabiola Zuluaga, to win three titles at the tournament. She saved a match point en route to the title, in the second round against Emina Bektas.