Novak Djokovic defeated Lorenzo Musetti in the final, 4–6, 6–3, 7–5 to win the singles tennis title at the 2025 Hellenic Championship. It was his 101st career ATP Tour-level title and his 72nd title won on hard courts, breaking a tie with Roger Federer. Aged 38 years and 5 months old, Djokovic became the oldest men's singles champion on the ATP Tour since its establishment in 1990, and the third-oldest men's singles tour-level champion in the Open Era.