The following are the seeded players. Seedings are based on WTA rankings as of 3 March 2025. Rankings and points before are as of 17 March 2025. Under the 2025 Rulebook, points from six of the seven combined WTA 1000 tournaments (which include Miami) are required to be counted in a player's ranking. }
Aryna Sabalenka ‡ † The player withdrew from the 2024 tournament but was not required to carry a 0-point penalty in her ranking due to a long-term injury exemption. Points from her 18th best result will be deducted instead. ‡ The player did not qualify for the 2024 tournament. Points from her 18th best result will be deducted instead.
Withdrawn seeded players The following player would have been seeded, but withdrew before the tournament began. † The player missed the 2024 tournament but was not required to carry a 0-point penalty in her ranking due to a long-term injury exemption. Points from her 18th best result will be deducted instead. == Other entry information ==