Defending champion Aryna Sabalenka defeated Amanda Anisimova in the final, 6–3, 7–6(7–3) to win the women's singles tennis title at the 2025 US Open. It was her second US Open singles title and fourth major singles title overall. Sabalenka was the first woman to defend the title since Serena Williams in 2014. She retained the world No. 1 singles ranking after reaching the quarterfinals; Iga Świątek and Coco Gauff were also in contention for the top ranking at the beginning of the tournament.