In enzymology, a 3α,7α-dihydroxy-5β-cholestanate—CoA ligase is an enzyme that catalyzes the chemical reactionATP + (25R)-3alpha,7alpha-dihydroxy-5beta-cholestan-26-oate + CoA AMP + diphosphate + (25R)-3alpha,7alpha-dihydroxy-5beta-cholestanoyl-CoA