Adenylyl-sulfate reductase

This enzyme belongs to the family of oxidoreductases, specifically those acting on a sulfur group of donors with other acceptors. The systematic name of this enzyme class is AMP, sulfite:acceptor oxidoreductase (adenosine-5'-phosphosulfate-forming). Other names in common use include adenosine phosphosulfate reductase, adenosine 5'-phosphosulfate reductase, APS-reductase, APS reductase, AMP, sulfite:(acceptor) oxidoreductase, and (adenosine-5'-phosphosulfate-forming). This enzyme participates in selenium metabolism and sulfur metabolism. == Mechanism ==

APS reductase catalyzes the reversible transformation of APS to sulfite and AMP, which is the rate determining step of the overall reaction. The reaction catalyzed by APS reductase is as follows: APS + 2e^- \longleftrightarrow AMP +HSO_3^- (E^\circ = -60 mV) The forward direction requires one AMP molecule; however, research suggests that the reverse reaction requires two AMP molecules (one acting on the substrate and one inhibiting the forward reaction). Dissimilatory sulfate reduction takes sulfate and transforms it into sulfide, a sulfur source that can be distributed throughout the body. Assimilatory sulfate reduction takes sulfate and turns it into cysteine. Dissimilatory and assimilatory pathways both use APS reductases as a metabolic tool to produce a sulfur source and amino acids, respectively. == Structure ==

As of late 2014, 6 structures have been solved for this class of enzymes, with PDB accession codes , , , , , and . The monomer of the enzyme consists of a mix of α-helices and β-sheets (both parallel and antiparallel). The protein cofactor thioredoxin can provide the required reducing equivalents for the reaction in the form of two cysteine residues, which are ultimately oxidized to a disulfide bond. The base active form of APS reductase appears to be a heterodimer, as seen in plants. In both bacteria and plants, two heterodimers tend to form together and produce a heterotetramer. The active site cleft in bacterial APS reductase has a few key elements. Residue sequences that appear to be necessary for catalysis are the P-loop (residues 60-66), the Arg-loop (residues 162-173), and the LDTG motif (residues 85-88). The P-loop, or phosphate-binding loop, is an especially important consecutive sequence of resides which aids in the recognition of the phosphate group in APS and, as a result, influences the substrate specificity for APS reductase. The C-terminal Cys256 is also catalytically essential, and seems to have a role in changing the conformation of the enzyme during catalysis. One notable chemical motif that distinguishes APS reductase from the related 3'-phosphoadenosine-5'-phosphosulfate (PAPS) reductase is the presence of a conserved cysteine motif, CC-X~80-CXXC, which occurs in addition to the universally conserved catalytic cysteine residue. This motif is correlated with the presence of a [4Fe-4S] cluster; therefore, these iron-sulfur clusters are not present within PAPS reductase. When the iron-sulfur cluster is present, it is required for catalytic activity and coordinated to the four cysteine residues in the conserved motif on the other side of the active site cleft. == Function ==

Sulfur is a vital component in biological life and a key element in amino acids cysteine and methionine. Biofoulants can contain a number of sulfate reducing bacteria, and studies have shown that if wastewater plants are left untreated sulfate levels will decrease. These studies have further solidified APS reductase's crucial role in the global sulfur cycle by giving organisms another unique way to obtain sulfur when it's unavailable. == Clinical significance ==

APS reductase does not exist within the proteome of human cells; consequently, these enzymes have become the targets of research for various environmental and medical reasons. Competitive inhibitors for the APS reductase in Mycobacterium tuberculosis have been studied as a new possible route for TB treatment, especially against drug-resistant and latent TB. Such inhibitors have also been studied in the context of obtaining oil and gas from reservoirs in order to better control the souring of such products. Some APS reductases have also been investigated for their role in selenium metabolism and reduction due to the chemical similarity between sulfur and selenium. APR2, the dominant APS reductase isozyme in the model plant Arabidopsis thaliana, has been implicated in the involvement of selenate tolerance and selenite metabolism. Such research may then aid in the goal of enhancing selenium phytoremediation in plants and, as a result, dietary biofortification. == References ==