"Ante Up" was featured in several films within years of its release, such as
The Last Castle,
Brown Sugar,
Dickie Roberts: Former Child Star, the dance film
You Got Served,
30 Minutes or Less and
Teenage Mutant Ninja Turtles: Mutant Mayhem. The song was also featured in an episode of the short lived television series
Robbery Homicide Division, as well as an episode of
Brooklyn Nine-Nine ("The Chopper"), an episode of
The Mindy Project ("In the Club") and an episode of
Superstore ("Mannequin"). In 2003, the song was sampled by
Javine Hylton for her debut single "
Real Things". It was later sampled by
John Cena in the song "
The Time Is Now". M.O.P. eventually sued, but later dropped legal proceedings for the alleged unlawful sampling. The guitar version of the song can be heard in the video game
Midnight Club 3: Dub Edition. In 2014, it was used for an advert for
O2 Bundles in the UK, and is used in British TV show
Soccer AM in a segment entitled "that's obscene, that is". In 2017,
YouTuber and member of the
Sidemen,
Wroetoshaw, sampled the song for his
KSI diss track
KSI Sucks, which, as of January 2023, has over 70 million views on
YouTube. In 2018, Wroetoshaw sampled the song again for his
RiceGum diss track, entitled
Ricegum Sucks. This has, as of January 2023, over 30 million views on YouTube. ==Charts==