The club was founded in 1965. As a result, the club were promoted to Division One East of the Hellenic League. After finishing fourth in their
first season, they claimed the runners-up spot in
2008–09 season, earning promotion to the Premier Division. The game also led to Ascot's then-record attendance of 1,149. In 2013–14 the club won the Hellenic League Challenge Cup, defeating
Ardley United 2–1 in the final. They won the Hellenic League's Floodlit Cup in
2015–16 with a 2–0 win against
Brimscombe & Thrupp in the final. The
2018–19 season saw Ascot win the Challenge Cup for a second time with a 3–1 win against
Windsor in the final. At the end of the season they were transferred to the Premier Division of the
Combined Counties League. Following league reorganisation in 2021, the club were placed in the Premier Division North of the league. In
2022–23 they were Premier Division North champions, earning promotion to the South Central Division of the
Isthmian League. The club also won the
FA Vase, defeating holders
Newport Pagnell Town 1–0 in the final at
Wembley Stadium. In
2024–25 Ascot finished fourth in the South Central Division, qualifying for the promotion play-offs, in which they lost 2–1 to
Hanworth Villa in the semi-finals. ==Ground==