In 1491, during his search for the Apple of Eden, stolen by
Girolamo Savonarola,
Ezio Auditore da Firenze is summoned by his ally Antonio de Magianis to
Venice to meet one of his contacts from Spain,
Luis de Santángel. Representing the navigator
Christopher Columbus, Luis asks Ezio to ensure the former's protection, fearing that someone is trying to assassinate him. Though initially reluctant, Ezio agrees to help after learning of Columbus's connections with the
Templar grandmaster,
Rodrigo Borgia. After foiling an attempt on Columbus's life, Ezio escorts him back to Luis, whereupon the two ask Ezio for a final favor: to recover an atlas from their hostel, which contains maps that detail travelling routes vital for their upcoming expedition. Ezio retrieves the atlas and brings it to Luis, who before leaving, reveals that most of the Spanish Assassin Brotherhood has been arrested by
Tomás de Torquemada as part of the
Spanish Inquisition. Returning to Antonio, Ezio announces that his search for the Apple will have to be postponed, because he is travelling to Spain to reform the Brotherhood there. In
Barcelona, Ezio searches for the local Assassins' headquarters, only to find it occupied by Inquisition soldiers. After escaping from the ambush, Ezio encounters an Assassin, Raphael Sánchez, who informs him that the rest of the Assassin Guild in the city were arrested on the orders of Gaspar Martínez. Ezio infiltrates Martínez's quarters and questions him about the captured Assassins. The inquisitor denies holding any Assassins prisoner, but admits that one of them is to be publicly executed. Ezio then kills Martínez, takes a list of names from his body, and saves the Assassin from being
burnt alive. Returning to Raphael with the list, he recognizes the names as being Assassins from
Zaragoza, and instructs Ezio to travel there and find Pedro Liorente, the Inquisition's calificador. In Zaragoza, Ezio witnesses one of the Assassins being executed on the orders of Liorente and Tomás de Torquemada, who are following the instructions of Rodrigo Borgia himself. After rescuing the remaining Assassins, Ezio assassinates Liorente, who denies the Templars' existence before dying. He then returns to Raphael, who reveals himself to be the Treasurer to
Queen Isabella and that the Assassins are fighting to stop the Inquisition, which is a Templar scheme to rid Spain of
Moors. Travelling to
Granada next, Ezio runs into Luis de Santángel again, who reveals that he too is an Assassin and is working at the King's court. As the Assassins begin to suspect that the Templars are planning their own expedition to uncover the
New World, Ezio agrees to help Luis find a Templar spy and a passage into the besieged Alhambra palace. In the process, Ezio discovers that the Templars have been keeping King
Muhammed XI captive to prolong the siege and drain the Queens' resources on the
ongoing war. Ezio ultimately rescues the King and delivers the news of his abdication. In retaliation for messing up the Templars' plan with the King, Tomás de Torquemada orders a new round of arrests and executions. Ezio prevents some arrests and assassinates the Inquisitor in charge of the operation, Juan de Marillo. Meanwhile, after hearing the news of the war ending, Christopher Columbus threatens to indulge other offers to sponsor his expedition if Queen Isabella doesn't act fast to give him an answer. Unsatisfied that the Queen is uncommitted to his cause, Columbus heads to
Paris to accept an offer from
King Charles. The Templars attempt to assassinate him before he reaches his destination, but Ezio saves Columbus's life again and persuades him to return to Queen Isabella, as she changed her mind after talking with Luis and Raphael. After Columbus successfully launches his expedition, Torquemada dispatches soldiers to kill Luis and Raphael. Ezio protects both of them before tracking down and confronting Torquemada. The Grand Inquisitor denies his ties to the Templars, and claims that he only helped Rodrigo Borgia because the latter is also a man of great faith, revealing Rodrigo is canditating to become
Pope. Before Ezio can kill Torquemada, the latter narrowly escapes. Reporting about his failed assassination attempt to the other Assassins, Ezio expresses his belief that Torquemada might not have been a Templar after all, and that his death would have probably caused more turmoil rather than ending it. ==Development==