The
thallus of
Astrothelium tetrasporum is , discontinuous, and consists of sinuose to rows of spherical to slightly flattened areas. These areas become locally almost like thick , mostly consisting of a
hyaline cortex up to 230 μm thick, somewhat shiny, covering areas up to 10 cm in diameter, and are olive-green in colour. Ascomata are (pear-shaped), measuring 0.7–1.3 mm in diameter, single, and immersed in the thallus. The wall is and up to 70 μm thick.
Ostioles are apical to eccentric, not fused, erumpent, brown, and chimney-like. The does not contain oil globules.
Asci contain four
ascospores each. Ascospores are hyaline, , , measuring 145–175 by 30–35 μm, with rounded ends. When the spores are young, they have a markedly thickened median
septum with two polar gelatinous caps approximately 5 μm thick. were not observed to occur in this species. The thallus surface of
Astrothelium tetrasporum is UV−, and the
medulla does not react to
potassium hydroxide (K−).
Thin-layer chromatography analysis did not detect any
secondary metabolites. ==Habitat and distribution==