; : The protagonist of the game. Ayesha is an herbalist who ran a small workshop with her grandfather and little sister Nio until her grandfather died and Nio goes missing. Despite being left alone, Ayesha remained upbeat and continued to run the workshop. One day, Ayesha sees an apparition of Nio by her supposed grave. Convinced that her little sister is still alive, Ayesha sets off on a journey to discover the means to save her. The knowledge of alchemy has been passed down in her family, but she does not recognize herself as an alchemist. ; : Ayesha's younger sister who disappears one day while out gathering herbs. After a long search by Ayesha and nearby villagers found no trace of the girl, she is assumed dead, and a gravestone is erected at the place of her disappearance. The truth is that she has been captured by an alchemy creation that has gone out of control and she is reunited with Ayesha after a battle against Yggdrasil. ; : An experienced alchemist from another country with keen interest in ancient times. He is a calm and collected gentleman in pursuit of knowledge. Keithgriff first encounters Ayesha on one of his visits to an ancient ruin and points the young girl towards alchemy as the key to saving her younger sister. In battle, Keithgriff does not use a weapon, instead relying on alchemy related attacks, which he summons from his magic ring. He fights Ayesha over a flask found in a village that all inhabitants have disappeared from, but he is valuable to Ayesha, though that he is not aware of it himself that he has come to help her. ; : An acquaintance of Ayesha who makes her living selling artifacts excavated from ruins. Despite being a woman, she is considered one of the strongest amongst the prospectors, a group consisting mainly of heavily built men in their prime. Regina is a sociable and reliable girl with a bunch of younger siblings to take care of. ; : A girl who comes from a family of witches. Described as being cute and wearing a black pointy hat, Wilbell is a genius when it comes to magic, but knows that she is a long way to be acknowledged by her great-great-grandmother, to whom she looks up. Despite fighting with a broom, Wilbell is still learning to fly as a proper witch. ; : A calm and collected swordswoman that acts as Marion Quinn' bodyguard. By Marion's request, she offers her assistance to Ayesha, who in return helps in her efforts to improve her cooking and make other friends, with dubious results. She used to be a criminal, but she was captured and then saved by Marion. There exist at least seven others with the same appearance as Linca, as well as whom all possess the same name. Their personalities are however different. ; : A hunter who wants to defeat a dragon and wear its scales as armor. Despite his stoic demeanor, he is very protective of his sister Nanaca, and watches over her from afar as she looks for pasture for their cows. Juris fights using a hatchet and crossbow. ; :An office worker from a large faraway city in a western country. Marion is tasked with gathering information about the area. She is small in size, but firm, and loves cute things. She captured Linca in the past, and Linca now serves as her bodyguard. She is also looking for Keithgriff, who committed crimes in their home country. When she realizes how important he is to Ayesha and his thoughts, she decides to let him go free. Her name is
romanized as Marion Quin in the Japanese-language version of the game. ; : An automaton constructed with alchemy. She is the librarian of a library that collects information about alchemy. She cannot express feelings very strongly, and her memory has begun to get worse. She searches for a book for Ayesha in the library's collection, and she begins to become more human-like in her behavior by interacting with Ayesha. After her new master Keithgriff orders her to no longer follow orders, she proposes to come along with him on the journey he is about to set out on. Her name is romanized as Odileia in the Japanese-language version of the game. ; : The manager of a company with great influence. He holds a great interest about the ancient times and collects relics that others may view as trash. ;
Yggdrasil : The guardian of life who was made through ancient alchemy. He complements Odelia, the guardian of knowledge. ; : The regent of the wind spirits. ; : An enthusiastic clerk who workes in a shop owned by Harry. She manages the shop by herself since Harry is busy with his personal interests. Her name is romanized as Merietta Muir in the Japanese-language version of the game. ; : A traveler with no employment. He is devoted to creating art by playing music on his bagpipes. ; : A nomad who, according to her family's customs, keeps cows and travels in search of pastures. She is Juris's younger sister. ; : A traveling salesman who is like a parental figure to Ayesha. ; : A girl who mines salt together with her family in a desert. ; :A bread-obsessed baker who has set up shop in Vierzeberg. He has a crush on Marietta, and changes moods drastically when she is around. ; :A barkeeper in Hornheim. He behaves in a very laid-back manner towards his customers and even rents out the room next to his tavern to Ayesha for use in alchemy. ==Atelier Ayesha Plus==