Prior to being occupied by Europeans, Aultsville and the surrounding area was home to many Native American settlements. When the Loyalists arrived, the land was divided and distributed with little thought given to the Native populations. The Natives were forced out of the area early into the settlement's history.
Loyalist settlement and the 18th century Aultsville, Ontario, was first settled in the late 1700s by a small group of five disbanded soldiers of the King's Royal Regiment of New York; these men were Loyalists who likely acquired the land here via grants for their loyalty to the Crown. Upon its initial settlement, the Loyalists called the settlement Charlesville, the origin of this name is unknown. By the end of the 18th century, Aultsville had already established its own post office with the first post master listed as one John Bockus. The post office remained in operation until 1957, when it closed for the flooding of the Seaway.
19th century – World War I By 1840, the population of Aultsville had risen to 120 individuals, and many more pioneer businesses and tradespeople were established in the area. The clay soil in the village allowed for brickyards and pottery works to be established. Many pioneer trades such as a blacksmith shop, shoemaker, carriage shop and tannery were also active in the village. Two more saw mills were also eventually erected in the village. The community was also home to an Oddfellows lodge, community hall and Masonic lodge. from the mid-1800s until the 1930s. During the mid-19th century, a small ferry operated in Aultsville in the
St. Lawrence River called the Aultsville Ferry. The ferry was a small, primitive wooden ferry which operated between Aultsville and Louisville Landing, New York during the summer months. Many disputes arose between the American and Canadian sides regarding the ferry, as it was in direct competition with the American ferry. Around 1860, Aultsville became home to its own school, referred to as the Aultsville Common School. This school served the elementary grades of the village. At some point closer to the 20th century, Aultsville established its own secondary school. This school is referred to in records as both Aultsville High School and Aultsville Continuation School. It is possible the school served as both at different periods; the difference between the two being that continuation schools served both elementary and secondary students. The secondary school and elementary school both operated until the 1950s, closing due to the flooding of the village. Aultsville High School had many sports teams including a hockey and baseball team, as well as a Cadet's Corps.
20th century By the turn of the century, Aultsville had added three more general stores, three cheese factories, another garage, four service stations, and a bank to its list of businesses. A grist mill in Aultsville was built around 1929. The mill contained metal grinding plates which ran on an electric motor; the mill mostly ground the local farmers personal grain supplies. In later years, the grist mill was used as an egg grading office. The first cheese factory was known as Croil's and McCullough's factory, and was located near the train station; cheese made here was shipped as far away as Montreal. Later this cheese factory became the Edward's Cheese Factory, and two more factories opened in the village both of which were also operated by the Edward family. These cheese factories were powered by steam, using coal-fired boilers to heat cheese vats. The cream for these factories was cooled and kept cool using a primitive cold-storage method; ice was cut during the winter months from the St. Lawrence River and insulated with sawdust. After World War II the village began to decline, many businesses closed and industries ceased operations. This was due to the fact roads in the area improved as had means of transportation; it was now feasible to travel to nearby communities for necessities. Additionally, many of the pioneer industries which once sustained Aultsville were becoming obsolete. By the mid-1950s, only one cheese factory, a bank, a
general store and the schools remained in operation. In 1958, the decision was finally made to construct the seaway, and the village was scheduled for demolition. The trees were cut from the town and the buildings of Aultsville burned in a controlled fire to test the effects of fire on buildings. Around July 1, 1958, Aultsville began to be flooded along with the other Lost Villages, the process taking around 4 days. ==The St. Lawrence Seaway Project and its effects==