In 2006, Aylesford were promoted into Championship 2 South East after finishing top of their previous league unbeaten. Also in 2006, their captain Natalie Garrett died of cancer. As part of a tribute, Aylesford Bulls have had NJG imprinted onto their playing shirts. They were almost relegated in 2010, which led to an overhaul in the way the team was managed. This included inviting
Germany women's national rugby union team head coach,
Susanne Wiedemann to coach them. The next year, they were promoted into Championship 1 after defeating
Plymouth Albion Ladies in a promotion playoff. The next season, they took part in the first Women's Kent Cup. In 2017, Aylesford Bulls Ladies won the final Women's Premiership before the introduction of the
Premier 15s league. Following this victory,
Harlequins Ladies took over the team fully and took their position in the new league. ==Notable former players==