SK Líšeň, playing in the Czech National Football League since 2019, was rebranded. After the 2024–25 season, the club changed its name to
SK Artis Brno and moved within the city of
Brno from the district of
Líšeň to
Královo Pole.
MFK Vyškov competed in the Czech National Football League from 2021 to 2025, but the club does not have a stadium that would allow it to play professional league matches, so it decided to play only in lower amateur tiers from 2025 and sold its place in the league to
FK Příbram. Team changes for sporting reasons included
SK Dynamo České Budějovice joining the league after being relegated from the previous season's First League, while last season's champions
FC Zlín changed places with them. Looking downwards, relegated teams
SK Sigma Olomouc B and
FK Varnsdorf were replaced by winners of the third tier leagues:
Ústí nad Labem, who won the
Bohemian Football League, and
Kroměříž, who won the
Moravian-Silesian Football League.
From CNFL •
FC Zlín (promoted to
2025–26 Czech First League) •
SK Sigma Olomouc B (relegated to
Moravian-Silesian Football League) •
FK Varnsdorf (relegated to
Bohemian Football League) •
MFK Vyškov (sold the league license to
FK Příbram)
To CNFL •
SK Dynamo České Budějovice (relegated from
2024–25 Czech First League) •
FK Viagem Ústí nad Labem (promoted from 2024–25
Bohemian Football League) •
SK Hanácká Slavia Kroměříž (promoted from 2024–25
Moravian-Silesian Football League) •
FK Příbram (bought the league license from
MFK Vyškov) ==Team overview==