Based on the locations where a majority of specimens have been found, being freshwater dominant and
tetrapod-bearing,
Barbclabornia is most likely a freshwater species, inhabiting
coastal plains of North America. The diet of
Barbclabornia can be inferred from its teeth, jaws, and size. Paleontologist Gary D. Johnson initially proposed that the shark could only bite down on its prey and swallow it whole. This would give it a diet of less active prey, primarily small
fish,
amphibians, and
arthropods. However, Johnson alongside
Jiri Zidek, William May, and Alvie Claborn would instead compare the large xenacanth with numerous tiny teeth to be
analogous to
modern filter feeding sharks, the
Basking Shark and
Whale Shark. This would indicate that
Barbclabornia was a filter feeder which would primarily consume
zooplankton. ==References==