In 1967, a pregnant woman is attacked by a vampire, causing her to go into
premature labor. Doctors are able to save her baby, but the woman dies. Thirty years later, the child has become the vampire hunter
Blade, who is known as the "Daywalker", a human-vampire hybrid that possesses the supernatural abilities of the vampires without any of their weaknesses, except for the requirement to consume human blood. Blade raids a
rave club owned by the vampire
Deacon Frost; a badly injured vampire is taken by police to the hospital, where he feeds upon
hematologist Karen Jenson and escapes. Blade takes Karen to a safe house where she is treated by his old friend
Abraham Whistler. Whistler explains that he and Blade have been waging a secret war against vampires using weapons based on their elemental weaknesses, such as sunlight, silver, and garlic. As Karen is now "marked" by the bite of a vampire, both he and Blade tell her to leave the city. At a meeting of pure-blood vampire elders, Frost, the leader of a faction of younger vampires, is rebuked for trying to incite war between vampires and humans. As Frost and his kind are not natural-born vampires, they are considered socially inferior. Meanwhile, returning to her apartment, Karen is attacked by police officer Krieger, who is a
familiar, a human thrall loyal to vampires. Blade subdues Krieger, but thanks to Karen, Krieger escapes. Blade tracks him down and beats him into revealing Frost's interest in an archive of ancient vampire knowledge. Krieger informs Frost of what happened and is killed for his failure. Frost also has Elder Dragonetti stripped of his fangs and torched alive by the sunrise, then strips the other Elders of their authority and imprisons them. Meanwhile, Blade visits the archivist Pearl, a morbidly obese vampire, and, with a UV light, tortures him into revealing that Frost wants to command a ritual where he would use twelve pure-blood vampires to awaken the "blood god" La Magra, and Blade's blood is the key. Later, at the hideout, Blade injects himself with a special serum that suppresses his urge to drink blood. However, the serum is beginning to lose its effectiveness due to overuse. While experimenting with the anticoagulant
EDTA as a possible replacement, Karen discovers that it explodes when combined with vampire blood. She manages to synthesize a vaccine that can cure the infected, but learns that it will not work on Blade. Karen is confident she can eventually cure his bloodlust, but it would take years he doesn't have. After Blade rejects Frost's offer for a truce, Frost and his men attack the hideout, where they bite Whistler and abduct Karen. When Blade returns, he helps Whistler commit suicide. When Blade attempts to rescue Karen from Frost's penthouse, he is shocked to find his still-alive mother, Vanessa Brooks, who reveals that she came back the night she was attacked and was brought in by Frost, who appears and reveals himself as the vampire who bit her. Blade, whose real name is revealed to be Eric Brooks, is then subdued and taken to the Temple of Eternal Night, where Frost plans to perform the summoning ritual for La Magra. Karen is thrown into a pit to be devoured by her colleague Webb, who has transformed into a decomposing
zombie-like creature. Karen injures him and escapes. Blade is drained of his blood, but Karen allows him to drink hers, enabling him to recover. Frost completes the ritual and obtains the powers of La Magra. Blade confronts Frost after killing all of his minions, including his mother, but initially finds him too powerful to defeat. Reasoning that Frost's physical body is still vulnerable, Blade gives him an overdose of EDTA; this triggers an adverse reaction that causes Frost to turn into a grotesque blob before exploding. Karen offers to help Blade cure himself; instead, he asks her to create an improved version of the serum so he can continue his crusade against vampires. In a brief
epilogue, Blade confronts an old vampire enemy in
Moscow. ==Cast==