New supporting cast Carlie Cooper – A lifelong friend of Lily Hollister, Carlie works as a forensics expert in the crime scene unit. New to her job, she shows interest in the more "exotic" cases, but has to start at the bottom. Her father is Ray Cooper, a deceased cop who was well respected by his peers. While preparing a body found in the river for the Medical Examiner, she finds a spider-tracer in its mouth, which initiates the police warrant for the "Spider-Tracer Killer" (believed by most to be Spider-Man). She is kidnapped by Dr. Rabin, an insane professor intent on using human sacrifices to appease an ancient Mayan god. He drags a bound and gagged Carlie out into a blizzard and attempts to murder her and offer her to the god, but she is saved when Spider-Man arrives and defeats Rabin.
Dexter Bennett – The new owner of the
Daily Bugle, which he renames the
DB. He has a personal vendetta against both Spider-Man and Peter Parker, which leads Peter and Robbie Robertson to leave the paper.
Lily Hollister – Harry Osborn's girlfriend. Her father, Bill Hollister, is running for Mayor of New York, chiefly at her insistence. As the supervillain
Menace, she is the most recent Goblin to become an enemy of Spider-Man, with a particular interest in the Mayoral elections.
Vin Gonzales – He works for the NYPD, and hates Spider-Man, thinking that the webhead is up to no good. However, his friend Al O'Neil, who also works for the NYPD, disagrees. Peter moves into Vin's apartment and becomes his roommate. When Peter resigns from the
DB, he does not want Vin to know, because he fears Vin would think he was rooming with a flake. When he does find out he is furious that Peter lied to him; however, they have since reconciled.
Other characters Ryan Maxwell – A construction worker who is knocked off a platform by Menace while changing the sign on the exterior of the
Daily Bugle building. Spider-Man arrives to catch Ryan, but Ryan's neck is injured in the process. Ryan later approaches the lawyer Matt Dowd, asking if he can sue Spider-Man for knocking him off a building. Dowd tells him that because his identity is unknown, they can file a "
John Doe" complaint and serve him by publication; if he doesn't respond, they get a default judgment against him.
Bruno Karnelli – The overweight son of a Karnelli and a Manfredi, who credits his parents' marriage with bringing the two crime families together, although he is offended that he has not been invited to their big meeting. He makes a deal with
Mister Negative, who promises to make Bruno head of both families. After Sean Boyle accidentally plants a spider-tracer on him, Spider-Man follows him (hoping it will lead him back to Boyle) as he is pulled into a blue van and kidnapped. Bruno is tied to a table by Mister Negative and his
Inner Demons as they extract a large amount of blood from him, until they are interrupted by Spider-Man, who lets them escape to save Bruno. Spider-Man believes that the only way Bruno would become leader of the crime families is if all other candidates for the job are killed, which makes Bruno realize that this has been Mister Negative's plan all along. Bruno then tells Spider-Man where to find them.
New villains Sean Boyle – This crook insulted Spider-Man by wearing a mask resembling his while committing crimes; he came to be known as the "Spider-Mugger". Boyle steals several wallets, including Peter Parker's and, accidentally, one of his Spider-Man webshooters. He attempts to sell the device to his fence, Dooley, at the Blind Spot (a waterfront bar for crooks), but Dooley considers the webshooter useless and returns it. When Boyle puts it on only to discover what it actually is, he accidentally shoots a spider-tracer onto the back of Bruno Karnelli, who is also at the bar. Boyle hides this from Dooley and starts using the webshooter to restrain his victims, until he realises that he must have stolen it from the real Spider-Man and one of the wallets he's given to Dooley contains his ID. He returns to the Blind Spot and asks for it to be returned, attacking Dooley with webbing, but Dooley turns it around on him and starts strangling Boyle with it. Later on, as Dooley drives off, Spider-Man finds Boyle's lifeless body with the webshooter and tracer still on him. Gonzales and O'Neil witness Spider-Man doing this and assume he has murdered Boyle.
Overdrive – Introduced in
Free Comic Book Day's Swing Shift, this villain can "
pimp out any ride", using nanites to control and reshape any vehicle he manages to steal. Claiming to be Spider-Man's biggest fan, he often remolds cars into replicas of his old Spider-Mobile.
Mister Negative – Martin Li, caring owner of a homeless shelter, has a dark secret: he can switch to a "negative mode", becoming a crime boss.
Freak – After ingesting drugs and the
Lizard's stem-cell research specimens, an addict morphs into a mammal-like being. Unlike the Lizard, Freak has his own mind when transformed and is obsessed with getting high. When nearly killed, Freak enters a metamorphosis state inside a cocoon and hatches in a significantly mutated form; he now cannot be defeated the same way as before.
Paper Doll – A teenaged girl who is obsessed with actor Bobby Carr. She dresses like a
goth, and has blue skin and the power to become paper thin and blend into backgrounds. She targets people who slander Bobby Carr and compresses them into her paper thin condition, thus killing them. ==In other media==