"Mr. Brightside" has been extensively used in media. In the
season 2 episode "The New Era" of the teen drama series
The O.C., which aired on December 2, 2004, the Killers play various songs from
Hot Fuss, including "Mr. Brightside", "Everything Will Be Alright", and "Smile Like You Mean It" during a scene involving a double-date in the Bait Shop. In the 2006 romantic comedy
The Holiday, Amanda Woods (portrayed by
Cameron Diaz) sets the CD player to play the song, then ironically dances to it and shout-screams the chorus while drunk and holding a glass of red wine, to avoid remembering the infidelity of her boyfriend Ethan (
Edward Burns). In the 2023 film
Saltburn, the song is briefly featured in a scene where Felix Catton (
Jacob Elordi) and Oliver Quick (
Barry Keoghan) are driving. Additionally, the song has been used in a variety of sporting events. The song has become a tradition among
Michigan Wolverines football fans at
Michigan Stadium, where fans in excess of 110,000 sing along to the tune when it is played in the fourth quarter. While the track was first played in an October 2016 game against Wisconsin, the tradition rose to prominence in 2017 when the crowd sang the second half of the chorus a cappella during a rain-soaked
Michigan State game. In the original release of
EA Sports College Football 26, the video game honors this tradition between the third and fourth quarters of games at Michigan Stadium. The song featured in the 2017
AFL premiership celebrations by
Jack Riewoldt of the
Richmond Tigers, who sang the song in its entirety live alongside the Killers. English darts player
Nathan Aspinall uses the song as his walk-on music, often resulting in sing-alongs with audiences. Starting in December 2023, the
Buffalo Bills play the song in the fourth quarter of home games accompanied by a lyric video. Additionally, videos were posted of the song and the team's reaction at Bills tight end
Dawson Knox's wedding to Alex Seefeldt in the summer of 2024. In July 2024, the Killers paused a concert during the
Rebel Diamonds Tour in London to allow the fans to watch the end of the
UEFA Euro 2024 semi-final match between England and the Netherlands, which England won 2–1 and advanced to the final. As the match ended, a series of red and white streamers were fired from cannons, with the band immediately launching into a rendition of Mr. Brightside. The band later posted on their
Twitter account that they "played for England". ==Accolades==