After earning her Ph.D., Culberson held a two-year NSF-funded research associateship in Chemistry at Duke before joining the Department of Botany as a Senior Research Associate. A botanical researcher at Duke University, Culberson pioneered the use of
thin-layer chromatography in the identification of secondary
lichen products, developing a standardized method that is still used today. She later refined this method and supplemented it with
high-performance liquid chromatography (HPLC). In 1969, Culberson published "Chemical and Botanical Guide to Lichen Products", a major work summarizing all published knowledge of
secondary metabolites made by lichens. She also produced two supplements to this guide. Culberson's research focused on characterizing
chemotypes, proposing
biosynthetic pathways of lichen secondary metabolites, and studying
gene flow between chemotypes. She developed skills in culturing single spore isolates and analyzing them with HPLC to search for evidence of gene flow between chemotypes. ==Personal life==