Production of microscopes in 1846 was a handcraft and art more than manufacturing enterprise. Each worker produced an instrument from start to finish without any division of labor. Early examples were even signed with the maker's name. Only such assemblies which were particularly time-consuming, such as the stage, were prepared in series in advance. The first moves to more efficient division of labor were made in 1857 when Zeiss separated optics under Löber from the metalwork of the stand.
Matthias Jakob Schleiden had been an interested patron and advisor since the founding of the firm, frequently spending hours at the workshops. He advised Zeiss to concentrate his efforts on the microscope which was critical for the rapidly advancing science of cellular anatomy and very much in demand. Schleiden had a personal interest as this was his field of study. As a result of the interaction, the first microscopes products of the workshop, the simple microscopes, were constantly improved. They were very favorably reviewed by the influential microscopist and botanist
Leopold Dippel (1827–1914). The optics for the simple microscope included a triplet of 200 fold magnification, for 5 Talers, and one of 300 fold magnification, for 8 Taler. These pushed the limits of the simple microscope. Greater magnification would require compound microscopes. Zeiss would need to expand his offerings so as not to be made irrelevant by his competitors. Production of compound microscopes required extensive research, which he had foreseen long in advance. Zeiss had developed into something of a bookworm in his limited spare time, researching everything available on theory of the microscope. He wanted, above all, to move past the prevailing methods of microscope production which relied on empirical matching of sets of lenses which would make up the high-magnification compound lenses he needed for compound microscope optics. Empirical methods used a selection of lenses, exchanging and examining elements, altering lens spacings again and again until a usable lens was obtained. Many dozens of lenses could be examined to produce the combination of three elements used in a microscope lens. A reasonably good lens obtained this way was altered and tried again and again to find the best result. To some extent, these designs could be reproduced, but each item was an empirical fit of the small elements which could not be reproduced exactly with the work methods used. Zeiss was, from the beginning, more a fine machinist than an optician. This meant that he was less constrained by the traditional work methods and thinking of contemporary opticians and more open to innovation. He decided to pursue the design of microscope optics by theoretical calculation, which expert opinion considered impossible for various reasons. Despite that opinion,
Joseph von Fraunhofer (1787–1826) had already produced telescope objectives by calculation in 1819, and
Josef Maximilian Petzval had done the same for the camera objective in Vienna with Johann Friedrich Voigtländer in 1840. Zeiss had already attempted to acquire the required theory in his evening literature studies. When this failed, he turned to the Jena professor of mathematics, Friedrich Wilhelm Barfuss, who had worked with his mentor Körner and had already worked successfully on the problem of Zeiss's simple microscope triplets. The collaboration continued until the professor's death, but offered no progress on the compound microscope problem. Zeiss's first compound microscopes were offered in his 5th, 1858, price list. These are described as "Small body tube, consisting of a field lens and two oculars with an adaptor to attach the tube to the stand and doublet objectives of stands 1 through 5 to allow use of the doublets as objectives to obtain two stronger magnifications after the fashion of the compound microscope. The 120 power doublet of the simple microscope yields in this fashion 300 and 600 fold magnification." Despite Schleiden's approval, these improvised compound microscopes were not a long-term solution. A similar arrangement, as a Brücke's Loupe, continued to be offered for many years with the dissecting stands but the original simple microscope doublets were an inferior substitute for a purpose designed compound microscope achromatic objective. By the publication of the 7th, 1861, price list in August 1861, newly developed compound microscopes appear in 5 different versions. The largest of these, costing 55 Taler, was a horseshoe foot stand as made popular by the well known Parisian microscope maker Georg Oberhaeuser. Under the object stage Zeiss introduced a domed aperture plate and a mirror mounted to allow not only side to side, but also forward movement to produce oblique illumination. Each microscope suite was produced to order for his customers so that they could choose their preferred optical components; objectives, oculars and illumination. The objectives for these new compound microscopes were still empirically design but nonetheless met with immediate approval from Leopold Dippel. Dippel examined the optical quality of the most useful objectives, A, C, D and F and had considerable praise for Zeiss's new objectives. The D objective was compared very favorably with the similar power objectives of Belthle and Hartnack (successor to Oberhaeuser). The F objective is even described as the equal of much more expensive objectives from established makers. It is assessed as almost as good as Hartnack's water immersion objectives. That was, of course the problem. When selling to researchers at the forefront of their fields, "almost as good" is a commercial disaster. Zeiss knew quite well that his strongest objectives could not match the quality of the Hartnack water immersion objectives. Every attempt to empirically design a satisfactory water immersion objective had failed. == Collaboration with Ernst Abbe ==