Ernst von Bergmann hired Schimmelbusch in 1889, having been impressed with his work on
bacteriology. Bergmann used a number of methods of disinfection and sterilisation, which Schimmelbusch documented and formalised. He referred to the equipment and methods used as the "aseptic apparatus", and in 1892, published
Anleitung zur aseptischen Wundbehandlung ("Guide to the aseptic treatment of wounds"), which became a seminal work on the subject. In Bergmann's obituary of Schimmelbusch, he wrote that the treatise "had been translated into almost all European languages and its procedures had spread worldwide." The procedures were heavily based upon the work of
Robert Koch and
Hugo Davidsohn, and combined Koch's technique of removing
anthrax spores to disinfect, along with hand sanitation methods proposed by
Paul Fürbringer and
heat sterilisation of surgical instruments. Although these practices were already used in laboratories, Bergmann and Schimmelbusch were among the first to apply them in a surgical setting. ==Death==