Macqueen played sarcastic consultant
anaesthetist Keith Greene in 75 episodes of
Holby City between May 2005 and July 2010. and also played Sir Jonathan Tutt, the British Ambassador to the UN, in the spin-off film
In the Loop; both were directed by
Armando Iannucci. He also acted in the
Comic Relief special of
Mr. Bean in 2007 as a vicar. He played
Kevin Sutherland in
E4's comedy,
The Inbetweeners. He appeared in the
Comedy Central /
BBC collaboration,
Kröd Mändoon and the Flaming Sword of Fire, In late 2009, he starred in several episodes of
The News at Bedtime on
BBC Radio 4, in a variety of supporting roles. In 2010, he appeared as the publisher Malcolm Dodds in
Woody Allen's
You Will Meet a Tall Dark Stranger. That year he also had a small role in the comedy film
Four Lions. In 2011, was in "
The National Anthem", an episode of the
anthology series Black Mirror. Macqueen had a lead role as Edmund in
Julia Davis's black period comedy
Hunderby, opposite Davis and
Alexandra Roach. In 2015, he also played the royal crier in
Cinderella. In 2016, he acted opposite
Michael Caine in the film
Youth, and played the role of Patrick Jarvis MP in series 3 of
Peaky Blinders. In 2019, he appeared in
Horrible Histories: The Movie – Rotten Romans and in 2022, he starred as
Stephen Gardiner in
Becoming Elizabeth. In 2023, he starred as Tindall in the
Apple TV+ series
Hijack, alongside
Idris Elba and
Neil Maskell. ==Filmography==