Bolton family •
Ripster (voiced by
Lee Tockar) – John Bolton is the smartest, the leader, and oldest of the four brothers who enjoys creating inventions. He has taken up most of his knowledge and skills from his father. He is also an avid
pool player. John was transformed into a
great white shark hybrid with the ability to able to bite through steel as well as having
extrasensory abilities. •
Jab (voiced by
Matt Hill) – Clint Bolton is the laziest of the Street Sharks, but enjoys
boxing and appears to have some talent with mechanics. Clint was transformed into a
hammerhead shark hybrid and uses his head as a battering ram. •
Streex (voiced by
Andrew Rannells) – Robert "Bobby" Bolton Jr. is a level-headed, cool, and self-proclaimed ladies' man. He is always shown wearing his
rollerblades and appears to enjoy parachuting and snowboarding and later plays drums professionally. Robert was transformed into a
tiger shark hybrid, deriving the Streex name from the purple streaks on his body. •
Big Slammu (voiced by D. Kevin Williams) – Coop Bolton is the strongest and youngest of the four. He is also a
football player in high school. Coop was transformed into a
whale shark hybrid and utilizes the "Seismic Slam", which enables him to shake or crack the ground with his fists. •
Dr. Robert Bolton (voiced by D. Kevin Williams) – A university
professor and the father of the Bolton Brothers who invents the gene-manipulation device for peaceful uses. He is only seen on-screen in the first episode where he tries to stop Dr. Paradigm's experiments, only to become changed into an unseen inhuman creature. Bolton secretly assists his sons, contacting them through a TV screen. •
Sir Thomas Bolton - An ancestor of the protagonists who is encountered in the episode "Sir Shark-a-Lot". He is the target of Paradigm's time-travel excursion to destroy the Bolton family.
Allies •
Lena Mack (voiced by Pam Carter) – A student of Dr. Paradigm's who suspects him of crime and therefore assists the Street Sharks. She has a younger brother named Malik. •
Bends (voiced by Jim Hoggatt) – Fission University's technical genius and a source of comic relief who supplies the Street Sharks with their motorbikes and weapons and conceals them underneath the University's ice skating rink. •
Moby Lick – Jets Taylor is a good friend of the Bolton Brothers and Bends. Under Dr. Paradigm's mind-control, Jets is combined with an
orca by Dr. Paradigm in order to make him his latest Seaviate. Becoming Moby Lick, he later broke free of Paradigm's mind control and became an ally of the Street Sharks. Moby Lick has a long prehensile tongue, superhuman strength, the ability to inhale water and expel it through his blowhole, and the ability to communicate with other killer whales. In the episode "Shark Hunt", Moby becomes an eco-conservationist in the
Everglades National Park. •
Rox (voiced by
Lee Tockar) – Melvin Kresnik is an up-and-coming musician who is mistakenly combined with a
bull shark and exposes Dr. Paradigm's plan to change Kresnik's audience into mutants. Thereafter, Rox continues as a rock star by explaining his altered form as a costume and remains a friend of the Sharks. •
David Horne (voiced by
Tony Wike) – The
President of the United States. The Street Sharks save him from Dr. Paradigm before he can be "gene washed" like he did with Vice-President Russell. Following this incident, Horne secretly leaks information to the Street Sharks. •
El Swordo (voiced by
Garry Chalk) – A circus performer who worked with a large
marlin named Spike. The two were combined by Dr. Paradigm, transforming El Swordo into a humanoid marlin. El Swordo remains active in entertainment as a professional swordsman. •
Mantaman (voiced by D. Kevin Williams) – A flying dinosaur-like alien specimen was found encased in stone by Dr. Terrence "Terry" Morton and reactivated by Dr. Paradigm. Dr. Morton then deliberately gene-slammed himself with a combination of the alien's DNA and
manta ray DNA to help the Street Sharks fight the original alien. •
The Dino Vengers – A military unit from an
exoplanet inhabited by mutant dinosaurs who later allied with the Street Sharks. Once ordinary members of their race, the four volunteered to receive genetic enhancements that made them far larger and stronger to better combat Bad Rap and his gang of terrorists. •
T-Bone (voiced by
Ian James Corlett) – A
Tyrannosaurus and the leader of the Dino Vengers. •
Stegz (voiced by D. Kevin Williams) – A
Stegosaurus. •
Bullzeye (voiced by Jason Michas) – A
Pteranodon. •
Spike (voiced by Garry Chalk) – A
Triceratops.
Antagonists •
Dr. Luther Paradigm (voiced by J. Michael Lee) – The main
antagonist of the series and arch-enemy of the Street Sharks. A
professor at Fission City University, he is easily identified by his metal
eyepatch and later by a giant yellow robotic exoskeleton, with offensive capabilities including the ability to fire
harpoons. In the second episode, Paradigm is injected with
piranha DNA meant for Bends. When in public, Dr. Paradigm wears a robe to hide his exoskeleton. •
SharkBot (voiced by Steve Gibbs) – A product of Dr. Paradigm's experimentation with robotics. It was used to free Repteel from prison and frame the Street Sharks for the crime. The Sharks ultimately reprogrammed SharkBot to destroy Paradigm's laboratory. SharkBot was later rebuilt as SharkBot 2.0, which fought the Street Sharks until the end of the series' second season. •
Tentakill – A bipedal creature of unknown origins and species, first seen in the episode "Sir Shark-a-Lot" as Paradigm's newest weapon. It is incapable of speech and possesses limited intelligence. •
Seaviates – A group of mutant sea creatures who serve Dr. Paradigm. •
Slobster (voiced by D. Kevin Williams) – A
lobster injected with the DNA of
Genghis Khan and
Thomas Blood. •
Slash (voiced by Terry Berner) – A
swordfish injected with the DNA of villains like Genghis Khan and Thomas Blood. Slash possesses a
drill bit on its nose and speaks in a hissing lisp. •
Killamari (voiced by D. Kevin Williams) – An anthropomorphic
squid that is able to project natural "spears" or "harpoons" from his mouth and the many suckers covering his body. •
Repteel (voiced by
Tony Wike) – The only one of Dr. Paradigm's Seaviates to have originally been human. Mr. Cunneyworth is the aged owner and
hotel manager of a run-down hotel that was accidentally demolished by the Street Sharks during a fight with Paradigm's Seaviates. As he had nowhere else to go, Cunneyworth willingly allows Paradigm to gene-slam him with a
moray eel and an
electric eel. As Repteel, he feeds on electricity and can shoot miniature eels (also charged with electricity) from his hands. •
Shrimp Louie (voiced by
Andrew Rannells) – A mutated
shrimp and one of Paradigm's later Seaviates. He is not very strong, is quite the coward and his primary weapons are large blaster guns. •
Maximillian Greco – An aged mafioso crime boss who blackmails Dr. Paradigm into using the Genetic Engineering Chamber to gene-slam him with the DNA of a
rhinoceros and
desert tortoise upon learning of his illegal and secret experiments. This granted Greco the strength of a rhinoceros and the longevity of a tortoise, rejuvenating him to half his real age. •
Zeus and Apollo (vocal effects provided by D. Kevin Williams) – Greco's pet
chihuahuas. They were genetically enhanced by Dr. Paradigm which made them larger than normal. •
Malcolm Medusa III (voiced by J. Michael Lee) – A rich businessman and
big game hunter who frequently targets endangered animals. •
Clammando (voiced by D. Kevin Williams) – Medusa's right-hand man. He started out as a
dock worker for Medusa before being mutated by toxic waste and transformed into an anthropomorphic
clam. •
Dr. Techno-Piranoid (voiced by J. Michael Lee) – A future counterpart of Dr. Paradigm. Techno-Piranoid first appears in the episode "Shark to the Future" when the Street Sharks visit his timeline, where he has taken over the world with an army of Seaviates. In "Shark Wars", Techno-Piranoid collaborates with his present-day counterpart to make sure an event that led to his future remains intact. When the Street Sharks foil the plot, the future is altered and Techno-Piranoid vanishes. •
MechaSharks – Dr. Tecno-Piranoid's army of mechanized shark monsters. •
The Raptors – A trio of rogue
Velociraptor-like criminals who come from the same exoplanet as the Dino Vengers. •
Bad Rap (voiced by
Doug Parker) – The leader of the Raptors who has a metal brace-like device on his mouth and a rocket launcher on his right hand. •
Haxx (voiced by Doug Parker) – A Raptor with implants on the backs of each wrist that produce green blades. His tail has been replaced with a blade capable of spinning like a drill. •
Spittor (voiced by Doug Parker) – The scientist and brains of the Raptors. Spittor carries a tank with various liquids released from nozzles on his hands, tail, and mouth.
Recurring characters •
Guy in the Sky (voiced by
Tony Wike) – Fission City's top air radio reporter and paparazzo. •
Detective Michael Brock – A police
detective who investigates the Sharks' activities. •
Mayor Moreno - The mayor of Fission City. •
Danielle Lafond - Fission City's news reporter. ==Series overview==