The club was established in October 1883 and were founder members of the Bedfordshire FA the following year. In their first competitive match they lost 4–3 to Luton Montrose in the Bedfordshire Senior Cup after being 3–0 up. and in
1956–57 they reached the first round of the
FA Cup for the first time, eventually losing 3–1 at
Margate. In 1961 Dunstable switched to the
United Counties League, but left after two seasons to rejoin the Metropolitan League, becoming one of only three first teams playing in Division Two. Although Best only played in two friendly matches and made no competitive appearances for the club, They remained in the division until the end of the
1993–94 season, after which they folded. They finished as runners-up in their first season, earning promotion to the Premier Division. They left the league in 1954, They went on to win back-to-back United Counties League titles in 1958–59 and 1959–60, but were demoted to Division Two in 1961 when the club's first team joined the league. In 1964 they returned to the Hellenic League, and were placed in Division One, but took over from the first team in the Metropolitan League for the 1965–66 season. They rejoined Division One of the United Counties League in 1967, but left at the end of the 1968–69 season. ==Ground==