Matthysse made her professional debut on 18 May 2007, scoring a four-round
unanimous decision (UD) victory against Graciela Adriana Becerra at the
Estadio Socios Fundadores in
Comodoro Rivadavia, Argentina. After compiling a record of 9–3–1 (1 KO), she challenged
Yazmín Rivas for the
IBF female bantamweight title on 4 February 2012, at the Gimnasio de las Liebres in
Río Bravo, Mexico. Matthysse failed in her first attempt at a world title, losing via UD over ten rounds. Following a UD victory against Antonia Ayala Vazquez in March, Matthysse faced former
two-weight world champion
Marcela Acuña on 10 May 2013, at the Auditorio Presidente Néstor Kirchner in
Tapiales, Argentina, with the vacant IBF Inter-Continental female
super bantamweight title on the line. Matthysse suffered the fifth defeat of her career, losing via ten-round UD with all three judges scoring the bout 99–91. She bounced back from defeat with a UD victory against Anahi Yolanda Salles in August, before suffering another defeat, losing via ten-round UD against
WBC Silver female
featherweight champion Betiana Patricia Viñas on 27 September, at the Polideportivo La Colonia in
Junín, Argentina. In her next fight she challenged
WBA female featherweight champion Ogleidis Suárez on 13 December 2013, at the Teatro Griego Juan Pablo Segundo in
San Martín, Argentina. Matthysse defeated Suarez via UD to capture her first world title, with the judges' scorecards reading 100–90, 99–91 and 97–93. She moved down a weight class in her next fight for a rematch with newly crowned
WBO female super bantamweight champion Marcela Acuña. The bout took place on 23 August 2014 at the Sociedad Alemana de Gimnasia de Villa Ballester in Jose Leon Suarez, Argentina. Matthysse suffered her second defeat at the hands of Acuña, losing via ten-round UD with the judges' scorecards reading 98–92, 97–93 and 96–94. After a UD victory against Lizbeth Crespo in a non-title fight in May 2015, Matthysse put her WBA title on the line in a
unification fight against
WBC female featherweight champion Jelena Mrdjenovich on 1 August in
Caseros, Argentina. After the ten rounds were complete, Matthysse emerged victorious via UD to become the new unified featherweight champion, with the judges' scorecards reading 98–92, 97–93 and 96–94. The pair would have a rematch on 11 March 2016, at the
Shaw Conference Centre in
Edmonton, Canada, with Mrdjenovich gaining her revenge, dropping Matthysse to the canvas in the tenth and final round en route to a UD victory. Two judges scored the bout 96–93 and the third judge scored it 97–82 to hand Matthysse the eighth defeat of her career. Following a UD loss against Natalia Vanesa del Valle Aguirre in June 2017, Matthysse suffered her third consecutive defeat, losing via UD against South American female super bantamweight champion Laura Soledad Griffa on 28 October 2017, at the Club Union Central in
Villa María, Argentina. She broke the losing streak with a UD victory against Anahi Yolanda Salles in August 2018, before defeating Cintia Gisela Castillo to capture the vacant
FAB female featherweight title on 6 July 2019, at the Club Social y Plottier in
Plottier, Argentina. Her next fight came against
WBO female junior lightweight champion Ewa Brodnicka on 4 October 2019, at the
Hala Sportowa Częstochowa in
Częstochowa, Poland. Matthysse suffered the eleventh defeat of her career, losing via
split decision (SD) with two judges scoring the bout 97–93 and 96–94 in favour of Brodnicka, while the third judge scored it 96–95 for Matthysse. Matthysse challenged
WBC and
IBO female super-featherweight World champion
Alycia Baumgardner at
Manchester Arena, Manchester, England, on 16 April 2022, losing the fight by unanimous decision with all three ringside judges' scorecards reading 100-90. She fought
Elif Nur Turhan for the vacant
WBF female
light-welterweight title at City Square in
Tetovo, North Macedonia, on 29 July 2023, losing via unanimous decision. Matthysse faced Samantha Worthington for the
WBA female super-lightweight championship at
Little Caesars Arena in
Detroit,
Michigan, USA, on 22 February 2026. She won when her opponent retired at the end of the eighth round, thus becoming a two-division champion. ==Professional boxing record==