Elizabeth Knollys was born on 15 June 1549, the second daughter and one of the 15 children of
Sir Francis Knollys and
Catherine Carey, the daughter of
William Carey and
Mary Boleyn. This made Elizabeth the grand-niece of Queen Anne Boleyn, second wife of
Henry VIII. She had 11 surviving siblings, the eldest of whom, Lettice Knollys, would later be banished from court after secretly marrying Queen Elizabeth's favourite,
Robert Dudley, 1st Earl of Leicester. She was brought up in a staunchly
Protestant household at
Greys Court at
Rotherfield Greys in Oxfordshire and Abbey House at
Reading in Berkshire. In 1556, three years after the
Catholic Princess
Mary Tudor had become queen, Sir Francis Knollys and his wife were compelled to seek refuge in Frankfurt, Germany to escape the relentless
Marian persecutions against known Protestants. It is not known if Elizabeth accompanied them as her parents took only five of their children abroad, leaving the others behind in England. Knollys went to court as a Maid of Honour to Queen Elizabeth shortly after the latter ascended the English throne in 1558. Her mother was Chief
Lady of the Bedchamber and her sister Lettice was a Maid of the Privy Chamber. Another sister,
Anne would later join the royal court. On 5 January 1566, Elizabeth Knollys was appointed a Gentlewoman of the Privy Chamber receiving an annual salary of £33 6s 8d. ==Marriage==