Eoholocentrum is an extinct genus of prehistoric marine ray-finned fish that lived during the early Eocene. It contains a single species, E. macrocephalum, known from the Early Eocene of Monte Bolca, Italy. It resembled and was closely related to modern squirrelfishes and soldierfishes, and appears to have been more closely related to squirrelfishes. It can be considered a basal or stem member of the Holocentrinae.