Hours after the order was signed, the Trump administration deleted mentions of LGBTQ+ resources across federal government websites. In April 2025, White House press secretary
Karoline Leavitt stated, "As a matter of policy, we do not respond to reporters with pronouns in their bios" in response to a journalist who queried her for unrelated information. She later stated, "Any reporter who chooses to put their
preferred pronouns in their bio clearly does not care about biological reality or truth and therefore cannot be trusted to write an honest story."
Department of State On January 22, 2025, Secretary of State
Marco Rubio directed the
Department of State to suspend all passport applications seeking a sex marker change or a nonbinary "X" sex marker. By January 23, 2025, a state department webpage describing how to amend the gender marker on passports was taken offline. According to a White House spokesperson, passports that have not expired will remain valid, regardless of how gender is depicted, but new applications will have to comply with the order and designate sex according to that assigned at birth. Some trans people also allege that upon applying to renew their passports, their documents were seized indefinitely and they were not issued a passport in any form. International travel advisories by the State Department replaced their language on "LGBTQ+ Travelers" with language around "LGB Travelers" and removed reference to issues unique to transgender travelers to other countries. On February 25, Rubio announced that transgender visa applicants who list a sex other than their assigned sex at birth on their visa application would be permanently banned from entry to the United States, and that applicants who do list their assigned sex on their application but whose home documents list a different sex would have their file marked with the letters 'SWS25' for tracking purposes. The announcement is framed as part of a ban targeting transgender female athletes alongside
Executive Order 14201, however according to legal experts, the actual text of the order would apply to all transgender travelers.
Department of Health and Human Services On February 1, 2025, the
Centers for Disease Control and Prevention ordered its scientists to retract any not yet published research they had produced which included any of the following banned terms: "Gender, transgender, pregnant person, pregnant people, LGBT, transsexual, non-binary, nonbinary, assigned male at birth, assigned female at birth, biologically male, biologically female".
Larry Gostin, director of the World Health Organization Center on Global Health Law, said that the directive amounted to censorship of not only government employees, but private citizens as well. For example, if the lead author of a submitted paper works for the CDC and withdraws their name from the submission, that kills the submission even if coauthors who are private scientists remain on it. All references to transgender people and gender identity were also removed from the
Centers for Disease Control's website, including survey results lessons on building supportive environments for trans and nonbinary students. The CDC and other federal agencies also directed their employees to remove pronouns from their email signatures. described in its background introduction as being "designed to help NIAID staff communicate with empowering rather than stigmatizing language", was also removed. On February 19, the
Office on Women's Health launched a website entitled "Protecting Women and Children", which featured a one-page explanation of the department's transgender policy, defining a person's sex as "an immutable biological classification" determined strictly by their reproductive function as either male or female, and featured a video of conservative activist
Riley Gaines explaining the new policy. On February 26, the Centers for Disease Control and Prevention announced it would stop processing transgender-related identity data. In March 2025, hundreds of
National Institutes of Health grants were terminated for including targeted keywords, including relating to LGBT people and/or gender identity in a biomedical context. In April 2025, at least 34 online archives placed a disclaimer on their sites that reads, "This repository is under review for potential modification in compliance with Administration directives" as a result of this executive order. In June 2025, the HHS eliminated from their 2026 proposed budget the "Press 3 option" for LGBTQ youth calling the
Suicide and Crisis Lifeline (9-8-8) to connect to specialized counselors. Later that month, the
Substance Abuse and Mental Health Services Administration (SAMHSA) announced that this service will end on July 17. When asked about the proposed budget cut, a spokesperson for the
Office of Management and Budget stated that the youth service was a "chat service where children are encouraged to embrace radical gender ideology". In April 2025, the Trump administration demanded that states receiving money for
sex education under the
Personal Responsibility Education Program (PREP), a program overseen by HHS, remove all references to gender identity, transgender and
non-binary people from the curriculum. , at least eleven states have complied with the demands, while 16 other states and Washington DC have filed a lawsuit. On October 27, 2025, the deadline given by the administration to comply or risk losing federal funding, U.S. district court judge
Ann Aiken ruled that HHS may not cut funding from states that do not comply.
Department of Justice The
Human Rights Campaign (HRC) and
American Civil Liberties Union (ACLU) said they had received reports that transgender women were being transferred to men's prisons, told they would be, or moved to solitary confinement. The Bureau of Prisons stopped reporting the number of imprisoned transgender people as a result of the order. It was also argued to violate the U.S. Constitution's Fifth Amendment's
Due Process Clause and Eighth Amendment's prohibition against
cruel and unusual punishment. On February 24, 2025, U.S. district judge
Royce Lamberth temporarily blocked the transfer of trans women in federal prisons to men's facilities, and the denial of their access to
hormone therapy. The temporary restraining order was in response to a lawsuit filed the previous week by three imprisoned trans women. In December, the DoJ eliminated policies designed LGBT prisoners from sexual assault. In February 2026, the
Federal Bureau of Prisons adopted new policies based on policies in Florida that mandated conversion therapy for trans inmates. Under the federal policy, trans inmates would have their hair cut short, be taken off hormone medication, and instead be put into psychiatric therapy and given psychiatric drugs.
Intelligence services On February 18, 2025, the
DHS Office of Intelligence and Analysis removed from their policy manual rules prohibiting the I&A from surveilling people based solely on their sexual orientation or gender identity. On February 26, Director of National Intelligence
Tulsi Gabbard fired more than 100 intelligence officers from the
National Security Agency for discussing in group chats topics relating to preferred pronouns, gender transition, and polyamory, which were deemed "sexually explicit" by investigators. The officers' security clearances were revoked shortly thereafter. In October 2025, pursuant to Executive Order , "Restoring Public Service Loan Forgiveness", the administration finalized revisions to the
Public Service Loan Forgiveness program. The rules expand the power of the Education Department to exclude organizations that engage in activities involving a "substantial illegal purpose", including
puberty blockers for
trans youth (referred to as "chemical castration").
Department of Housing and Urban Development On February 7, the
Department of Housing and Urban Development declared that they would stop enforcing a 2016 policy prohibiting gender identity discrimination in housing programs and shelter spaces. According to an announcement by HUD Secretary
Scott Turner, the goal of this repeal was to allow women's shelters to implement bans on transgender women from entering. In November, the Trump Admin released new rules allowing them to deny funding to any homeless housing program or shelter that "violates the sex binary".
Department of the Interior The
National Park Service (NPS) removed all references to the existence of transgender people and transgender rights from its webpages covering the
Stonewall National Monument, the
Stonewall riots, and LGBTQ+ history more broadly, going so far as to change the acronym on the site from "LGBTQ+" to "LGB". NPS similarly altered the acronym on a webpage for the
Pauli Murray Family Home in February 2025, and, in addition, blocked the public view of an NPS biography on
Pauli Murray which had stated, "we do recognize that pronouns matter".
Department of Veteran's Affairs In June 2025, the
VA imposed new guidelines on its hospitals, citing this order in allowing hospitals to refuse to hire or treat individuals based on their political affiliation, marital status, and/or worker's union involvement.
Department of Homeland Security In February 2025, the
Transportation Security Administration issued a policy barring transgender officers from conducting or witnessing security patdowns. In September 2025,
U.S. Customs and Border Protection issued new regulations by which they would deny passage in their Advanced Passenger Information System to any passports bearing a non-binary gender marker.
Independent federal agencies The
National Endowment for the Arts has announced that all 2026 projects must ensure that they are "compliant with all legal, regulatory, and policy requirements applicable to [their] award". This includes not promoting "gender ideology". The
National Science Foundation compiled an internal list of words the presence of which in a research paper, grant application, or other relevant documentation, would flag a project and put its funding under review. Words that would initiate a review included "gender", "LGBT", and "women", among others.
NASA took down webpages relating to LGBTQ+ employee resource groups and diversity at the organization, and according to employees, verbally informed its employees that any display of LGBTQ+ symbols, such as a pride flag in one's workspace, would be met with being placed on administrative leave. Shortly following the order, the
Equal Employment Opportunity Commission's acting chair said that it would no longer allow "X" gender markers for those filing discrimination charges. By December, the EEOC had successfully thwarted almost every discrimination claim brought by a trans worker - with the few remaining being those pursued entirely through private lawyers. In February 2025, the
Office of Personnel Management (OPM) announced that insurance carriers that provide health insurance coverage to federal employees under the
Federal Employees Health Benefits Program must provide only two options for a person's sex (i.e., male and female) on their insurance forms. In July of that year, the OPM issued a memorandum giving all federal departments until August 11 to revoke any trans-inclusive policies and present a document to the OPM outlining how they did so. The
Social Security Administration removed its pages on gender identity and changing sex identification. It has not responded to requests for comment on its current policy. An internal message sent on January 31, 2025, instructed employees not to accept or process changes to gender markers on Social Security records, effective immediately. In August 2025, the OPM stated in an internal memo that beginning in 2026, gender-affirming care would no longer be covered by the
Federal Employees Health Benefits and Postal Service Health Benefits programs.
Non-governmental organizations On February 7, 2025, the
National Center for Missing and Exploited Children announced that they would remove all references to trans people from their public-facing materials in order to comply with the executive order. Shortly thereafter, the
Rape, Abuse & Incest National Network likewise cut mention of transgender people from its public facing materials. == Legal challenges ==