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Explant culture

In biology, explant culture is a technique to organotypically culture cells from a piece or pieces of tissue or organ removed from a plant or animal. The term explant can be applied to samples obtained from any part of the organism. The extraction process is extensively sterilized, and the culture can be typically used for two to three weeks.

Application
Historically, explant culture has been used in several areas of biological research. Organogenesis and morphogenesis in fetus have been studied with explant cultures. Since the explant culture is grown in the lab, the area or cells of interest can be labeled with fluorescent markers. These transgenic labels can help researchers observe growth of specific cells. For example, neural tissue development and central nervous system regeneration have been studied with organotypic explant culture. Even with all the advantages to explant culture, there still are several caveats. The downside of explant culture is that it does not provide sufficient time to study chronic diseases. Although two to three weeks may be enough time to study acute changes, it is not fit for experiments requiring long-term observations. ==Current research==
Current research
Retina Many neurobiological processes have been studied with retinal explant cultures. Understanding retina's development has led the way for researchers to study pathological neurodegeneration and related retinal diseases more closely. Cellular grafts derived from retinal stem cell therapy is an active area of research to treat macular degeneration, retinitis, pigmentosa, and glaucoma. ==References==
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