Gladstone Line trains begin at
Hoboken Terminal, with the exception of two weekday trains which run in and out of
New York Penn Station. Gladstone Line trains run over the Morris & Essex from Hoboken or New York to
Summit. At Summit, schedules are timed for most Morristown trains and Gladstone trains, permitting easy transfers across the platform or one right after the other. The Gladstone Branch parallels the Amtrak Northeast Corridor and
PATH lines and
Interstate 280 (I-280) for a short distance here. The
Waterfront Connection is just prior to the overpass at Meadows interlocking. It allows selected
North Jersey Coast Line and
Raritan Valley Line trains to access Hoboken from the Northeast Corridor Line. over the
Passaic River bridge. The swing bridge is to the right of the vehicular
William A. Stickel Memorial Bridge (I-280) lift bridge in background The line begins its journey by following I-280 and crosses a two-track
swing bridge over the
Passaic River. It enters
Newark Broad Street station, which features two high platforms serving all three tracks. Within the city limits of Newark, the line runs in a trench, passing beneath numerous streets, I-280, and the
Newark City Subway. At the site of the former
Roseville Avenue station, now known as the Roseville interlocking, the
Montclair–Boonton Line diverges to the north. The section of the track extending westward from the Passaic River to just east of Millburn station is composed of triple tracks, while the remainder of the route to
Lake Hopatcong station is a
double-track railway. After passing the abandoned station at Grove Street, now the location of Green interlocking, the line crosses the
Garden State Parkway and reaches
East Orange, which is situated on a viaduct. Subsequent elevated stations include
Brick Church and
Orange. The line then curves southward over Interstate 280, passing past
Highland Avenue and
Mountain Station. The next station is
South Orange, an elevated structure with two platforms and three tracks, near
Seton Hall University.
Maplewood follows, with a side platform and a center platform serving all westbound and some eastbound trains. Beyond Maplewood, the line narrows to two tracks at Millburn interlocking.
Millburn and
Short Hills stations each have two side platforms serving two tracks. Just west of Summit, the Morristown Line separates. The Gladstone Branch curves left at the Summit substation before entering
New Providence. After New Providence, the line passes its first grade crossing at a 4-way intersection of Central Avenue and Livingston Avenue. This crossing is one of the few 4-way crossings along NJ Transit rail lines. The line then continues, crossing Foley Place and entering
Murray Hill station. At Murray Hill, the track splits in two to allow eastbound trains and westbound to pass each other, since most of the line is single tracked. There is a wooden plank at Murray Hill to allow passengers on westbound trains to get off before the eastbound train arrives on the track closest to the station platform. Schedules are timed for a convenient meetup at Murray Hill. After Murray Hill, the line passes three more grade crossings: at Warner Field Path, at Union Avenue, and at Snyder Avenue. There used to be a siding for the General Chemical Company plant just west of the Snyder Avenue crossing, but this was removed between 2018 and 2019. The line crosses Plainfield Avenue and enters
Berkeley Heights station, which also contains a siding for parking equipment. The line then continues and crosses the
Passaic River a second time and enters Morris County. There are three stations in
Long Hill Township. The first is
Gillette station. After Gillette, the line crosses Morristown Road and enters
Stirling station. At Stirling, the track splits in two, again to allow eastbound and westbound trains to pass each other. The line also crosses Central Avenue at the station. Continuing westward, the line crosses Northfield Road by Millington Elementary School before curving right, crossing Division Avenue, and entering
Millington station. At Millington, there used to be another gateless crossing on the other side of the platform, where the line will cross River Road, a small connection road between Commerce Street and Long Hill Road, but this crossing was closed off sometime around 2014-2015. The line continues westward, crossing the Passaic River a third time before entering Somerset County and
Bernards Township and crossing Pond Hill Road. After this crossing, the Millington quarry can be seen on the left (going westbound). Here, there is a short passing siding; although trains going opposite directions can also pass each other here, this area is not usually used for that.
Lyons station is the next stop. The line enters the station after crossing a bridge over South Finley Avenue. After another crossing at Lake Road near Ridge High School and another bridge over West Oak Street, the line enters
Basking Ridge station at the crossing at Ridge Street. Until recently, the original semaphore signal from the DL&W years used to sit atop the Basking Ridge station over the platform, one of the last vestiges of the previous operator of the line. Afterwards, the line crosses over
Interstate 287 before curving left and entering
Bernardsville station after crossing under Mount Airy Road. At Bernardsville, the track splits in two, again to allow trains to pass each other if necessary, and the line crosses Depot Place. Continuing westward, the line parallels
U.S. Route 202 and crosses Old Quarry Road, Meeker Road, and Whitenack Road. At Whitenack Road is the site of the former Mine Brook station, a flag stop in the line's DL&W years. Mine Brook is the only station that was never rebuilt or even acquired by New Jersey Transit. Today, all that is left of the station is gravel where the platform used to be and a small gravel road where the parking used to be. The line then crosses Route 202 and enters Far Hills station, which has a station depot and a freight house to the west. Just west of the station near the freight house, the track splits in two again to allow eastbound and westbound trains to pass each other. After Far Hills, the line snakes through the Far Hills area before entering the borough of
Peapack and Gladstone while crossing over the
North Branch Raritan River and under Peapack Road.
Peapack station is next at the crossing at Holland Avenue. The line then crosses a walkway in Liberty Park before entering Gladstone yard. At Gladstone yard, the track splits into 5 to allow for parking of trains. One of the tracks switches back to condense into one or can act as a siding, while the other 4 tracks condense back into one. West of the yard, the tracks enter
Gladstone station and its freight house. One track leads to the freight house, while two lead to the station. The timetables are arranged so that most of the trains meet at Far Hills and Murray Hill. While Bernardsville and Stirling each have two tracks at the station, the second track at those two stations is less commonly used unless a train is running late. ==Freight service==