The club was founded on September 5, 2014, after the merger of the
Club Águilas Reales de Zacatecas and the reserve team of
Pachuca in the
Tercera División de México, the Zacatecan team gave up their rights to participate in the
Segunda División de México while Pachuca contributed the sports part of the organization. Previously in the Summer 2014 tournament, the city of
Tlaxcala had been left without a football team due to the relocation of
Linces de Tlaxcala to
Acapulco,
Guerrero, where the team was renamed as
Internacional de Acapulco, while Águilas Reales de Zacatecas was moved to
Pachuca,
Hidalgo and merged with the reserve team of Pachuca, which was later moved to the city of Tlaxcala giving birth to Tlaxcala F.C., and became a secondary team due to the arrival of
Mineros de Zacatecas, a team that shared the same ownership with Tlaxcala F.C. until 2020. Tlaxcala won automatic promotion to the
Ascenso MX after winning the two season tournaments against
Irapuato during the
2016–17 season, but their promotion was put on hold until before the 2018–19 season as their stadium failed to meet league requirements. They played in the
Serie B for the 2017–18 season so they can met requirements to play in the Ascenso MX, but their spot was revoked after the stadium was not completed at the deadline to meet the requirements, so they moved to
Serie A for the 2018–19 season. In 2018–19 season, the team played as local at Unidad Deportiva Próspero Cahuantzi at
Chiautempan, for the 2019–20 season, they moved to the Unidad Deportiva José Brindis in
Nanacamilpa awaiting the end of the construction works of the
Estadio Tlahuicole. In July 2020, Tlaxcala F.C. was invited to the new
Liga de Expansión MX, which became the second category of Mexican football instead of the Ascenso MX, with this the team occupied its place in the division after three years of having achieved sports promotion. On August 19, 2020, the club debuted in the Liga de Expansión, defeating
Mineros de Zacatecas 1–2. On September 2, Tlaxcala received
Celaya F.C. in its first home game, which had to be played in Nanacamilpa because the Estadio Tlahuicole was not yet ready to host professional football matches, and finally, on September 15, Tlaxcala was able to return to its stadium in the match against
Leones Negros UdeG, which was won by the
Guadalajara team. ==Personnel==