Woolston was still under refit at Chatham on the outbreak of the
Second World War. Around this time her
pennant number was changed to L49, to match those used by escort destroyers. She spent October on post refit trials and then commissioned for service, joining the
Nore Command in November to commence convoy defence duties in the
English Channel and the
North Sea. She continued these duties for the rest of 1939 and all of 1940. She was detached for a period in 1941 to cover Atlantic convoys as they passed through the
Western Approaches. In February 1942
Woolston was reassigned to the
Home Fleet and sailed to
Scapa Flow. After a successful '
Warship Week' in March 1942 she was adopted by
Congleton,
Cheshire. In March she was one of a number of destroyers screening heavy fleet units covering the passage of the
Arctic convoys PQ 12 and the returning
PQ 8.
Woolston then returned to the Nore Command and spent the rest of the year deploying in the North Sea.
Woolston was part of the fleet that put to see in July 1942 in an attempt intercept the
German battleship Tirpitz. She continued in these duties until May 1943, when she was assigned to cover military convoys passing through the Atlantic carrying troops and supplies for the
allied invasion of Sicily. She sailed to the
Clyde on 20 June and joined the outbound military convoy WS-31 on passage to
Gibraltar. She and the other escorts were detached from the convoy on its arrival in Gibraltar on 26 June.
Woolston then took passage to
Bône in early July and was nominated to join the Eastern Support force in Escort Group V. She left Bône on 7 July as part of the military convoy KMF18 on passage to the beach head, and was detached on 9 July to refuel in
Malta. She returned the next day to join the Escort Group in providing anti-aircraft defence of the anchorages off the beach head. On the successful completion of the landings
Woolston returned to the Nore Command and spent the rest of 1943 on convoy defence duty in the North Sea. She remained at this task for the rest of the war. ==Postwar==