Houlton is an unincorporated census-designated place located in the town of St. Joseph, St. Croix County, Wisconsin, United States. As of the 2020 census, Houlton had a population of 377. It is along Wisconsin Highways 35 and 64, Main Street, Hawk Street, and County Road E. Houlton is located across the St. Croix River from Stillwater, Minnesota. Houlton has no post office but has been assigned the ZIP code 54082.