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IKZF2

Zinc finger protein Helios is a protein that in humans is encoded by the IKZF2 gene. This protein is a member of Ikaros family of transcription factors.

Structure
Ikaros family members are characterised by having 4 N-terminal zinc finger domains, except for Pegasus, which has only 3. These are the key domains for DNA binding and stabilization of DNA-protein interactions. They also have C-terminal zinc finger domains which serve as a site for interactions with other proteins and hetero- or homodimerization with other family members. == Function ==
Function
As transcription factor Helios is said to repress the IL-2 expression in Tregs. This function was also confirmed for Eos, another member of Ikaros family of transcription factors, pointing to their redundant functions. Helios interacts with Foxp3 to lower IL2 expression. They form a complex and bind to the IL2 locus causing repressive epigenetic modifications, namely reduced histone H3 acetylation. IL-2 maintains Helios expression. IL-2 is probably not the only factor positively affecting Helios expression. We can divide Treg cells into two main subsets: thymus-derived Treg cell (tTreg) and peripherally-induced Treg cell (pTreg). TTregs are the subset of cells that develops in thymus from T lymphocytes that recognise self-antigens. Whereas pTregs are lymphocytes that are induced in the periphery originally from CD4+ Foxp3- cells and which subsequently acquire suppressive function. Both Treg cells subsets are Foxp3+. It was discovered that Helios is not essential for early development of T cells in thymus, but it is important for Treg suppressive function later in their life. This conclusion was drawn when the loss of Helios in T cells did not have any effect on T cell development and homeostasis of the immune system in a mouse model. However, it did result in a defective immune regulation later in the life with the onset of autoimmunity resulting from defective Treg function. Thus, this suggests that Helios is important for the identity of Treg cell and stability of their cytokine profile. It is important to mention, that so far we do not have enough knowledge about the mechanism of Helios keeping Treg stability and even about Helios's own expression in Tregs. Thus, there is a need to uncover the mechanism behind these findings. In other immune cells Beside CD4+ Tregs, Helios is also expressed in murine NK cells. In this immune cell subset, Helios is expressed early during their development, and it is later downregulated. Helios is also expressed in CD8+ regulatory T cells. Helios maintains their suppressive function similarly to the CD4+ Tregs. == References ==
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