A total of 20 teams take part in the league: 17 teams from the
2024–25 Eerste Divisie and 3 teams relegated from the
2024–25 Eredivisie.
Almere City replaced 2024–25 Eerste Divisie champions
Volendam after two seasons in the
Eredivisie.
RKC Waalwijk replaced second-placed
Excelsior after six seasons in the Eredivisie.
Willem II replaced
Telstar following their loss in the promotion play-offs after one season in the Eredivisie. On 31 July 2025, it was confirmed that
Vitesse had lost their professional license due to financial issues, and therefore the club was not eligible to play in the Eerste Divisie. The KNVB announced that no other team was eligible to be promoted, and as a result the competition began to be played with 19 teams. Period titles would have been decided on the first 8 or 9 matches each team played within a period, depending on whether such a period would have had 9 or 10 match days. On 3 September 2025, Vitesse won an appeal and regained their license, requiring them to be placed back in the league, and returning the league to the original 20-team rules.
Stadiums and locations Number of teams by provinces Personnel and kits Managerial changes == League table ==