James Gillespie may refer to:James Gillespie, U.S. Congressman from North Carolina James Gillespie (minister) (1720–1791), Scottish minister and theologian James Gillespie (footballer) (1868–1932), Scottish footballer James Gillespie (philanthropist) (1726–1797), Scottish snuff and tobacco merchant James Gillespie (mariner), HM Excise officer and commander of the cutter Henry Dundas James J. Gillespie (1892–1959), member of the Iowa Senate James U. Gillespie, member of the Florida House of Representatives