James Lamar Wittliff also known as Jim Wittliff, is an American Biochemist and Professor Emeritus of Biochemistry & Molecular Genetics at the University of Louisville. He was Chairman of the Department of Biochemistry at the University of Louisville School of Medicine and Dentistry from 1976-1983. Wittliff's laboratory was designated the National Reference Facility by the National Cancer Institute for performing quality assurance surveys of receptor biomarker testing in breast cancers for cooperative clinical trial groups in the United States and Canada. He is the co-developer with NEN/DuPont of the 1st FDA approved tests for quantifying levels of estrogen and progestin receptor proteins.