Japanese squirrels are diurnal and active throughout the year. They are solitary as adults, with minimal overlap between home ranges. Males typically defend a territory of , while females defend territories of . Territories tend to be larger in heavily fragmented environments, as squirrels need to incorporate larger quantities of less-suitable marginal habitat. The Japanese squirrel's predators include the
Japanese marten,
foxes, domestic
cats, domestic
dogs, and predatory birds including raptors and
crows. In certain areas, up to 35% of the Japanese squirrel's diet can come from walnuts. It is possible the resulting dispersion has affected the evolution of larger seed sizes among Japanese walnut populations where Japanese squirrels are present.
Reproduction Japanese squirrels are
polyestrous and go through two distinct reproductive pulses over the year, an initial one from February to March and a later one from May to June. Females undergo a single day of
estrus, during which they are followed and courted by multiple males. After mating, gestation lasts for 39 to 40 days, followed by the birth of a litter of two to six young are born within a leaf nest or den cavity. The young are weaned within a few weeks and afterwards disperse from their natal area. ==Conservation==